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Bacterial Overgrowth of the Small Intestine Breath Test -3 Hours-Genova Test Kit

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The 3 Hour Bacterial Overgrowth of the Small Intestine Breath Test is a Non-invasive, gastrointestinal test detects bacterial overgrowth in the small intestine, often referred to as BOSI or SIBO.


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A week before testing avoid the use of laxatives, restricted diet the day before (please refer to kit instructions for detailed information regarding important prep before starting test), and 12 hours fasting prior to the test. Ship to lab Monday-Thursday only. Please read all of the directions and the collection procedures prior to starting test.

Test Results:

10-12 Days once the lab receives the specimen. May take longer based on weather, holiday or lab delays.


This test uses lactulose; since lactulose contains galactose and lactose, it is not recommended for individuals who have had allergic reactions to lactulose, or who are on a galactose/lactose - restricted diet. It should be used with caution in diabetics. 

This simple, non–invasive, gastrointestinal test detects bacterial overgrowth in the small intestine, often referred to as BOSI or SIBO. Small intestine bacterial overgrowth (SIBO) is a common gastrointestinal disorder that often underlies chronic gastrointestinal symptoms of maldigestion and malabsorption, including bloating, gas, diarrhea, irregularity, and abdominal pain.

The 3-hour SIBO profile provides insight into gas levels over a longer period of time, and is recommended for patients with slower gastrointestinal transit or constipation.

Bacterial overgrowth can inhibit nutrient absorption and lead to the following serious health problems:

  • Altered intestinal permeability (leaky gut)
  • Carbohydrate intolerance
  • Malabsorption
  • Malnutrition and weight loss
  • Anemia
  • Fibromyalgia
  • Chronic Fatigue Syndrome
  • Osteoporosis


Causes of small intestine bacterial overgrowth (SIBO/BOSI):

  • Crohn's Disease
  • Adhesions
  • Radiation Damage
  • Lymphoma


Normally, far fewer bacteria inhabit the small intestine than the ample growth found in the colon. Gastric acid secretion and intestinal motility keep the small intestine relatively free of bacteria. However, a wide range of abnormalities and malfunctions can encourage bacteria to multiply in the small intestine causing SIBO/BOSI. The most common causes of SIBO/BOSI relate to a decrease in the production of hydrochloric acid or pancreatic enzymes, thereby creating an unsterile environment for the small intestine.

The Bacterial Overgrowth of the Small Intestine Breath Test (SIBO/BOSI) is appropriate for patients who demonstrate:

  • Irritable Bowel Syndrome (IBS), the most commonly diagnosed gastrointestinal disorder, affecting 20% of the general population. The SIBO test aids in identifying the actual cause of IBS.  Studies suggest that as many as 50-75% of patients with IBS have SIBO/BOSI, as demonstrated by hydrogen breath testing
  • Unexplained abdominal symptoms, especially gas and bloating
  • The inability to tolerate sweet or starchy foods, fiber, or friendly flora supplements

• Specimen Requirements: Eight fasting breath samples—at baseline, 20 min, 40 min, 60 min, 90 min, 120 min, 160 min, 180 min

• Before Patient Takes This Test: - Avoid high-fiber foods and supplements (2 days before) - Eat a light dinner (on the night before) -  Fast (12 hours before) -  Inform practitioner of all medications and supplements - See instructions inside test kit for details


You will receive an activation card with the test kit. To activate your test kit scan the barcode or go to gdx.net and follow the prompts.  

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