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Hormone Trio Blood Spot - ZRT Test Kit

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The Hormone Trio test evaluates three of the most popular hormones tested into one profile.


Sample Report Compare
Test Code:




Blood Spot


No fasting required. Specimen must be sent to lab by overnight mail Monday-Thursday only. Please read patient instructions very carefully and decide the ideal day for you to begin test.

Test Results:

5-7 Days once the lab receives the specimen. May take longer based on weather, holiday or lab delays.

The Hormone Trio helps classify different imbalances in the hormones that are most frequently examined. Hormone imbalances can cause various health problems. This test includes Estradiol, Progesterone, and Testosterone testing and is an effective assessment for both males and females.

Females may consider this test for:

  • Baseline levels before hormone replacement therapy
  • Ideal for monitoring HRT dosing
  • Amenorrhea
  • Menopausal symptoms
  • PMS
  • Anovulation
  • DUB (dysfunctional uterine bleeding)
  • PCOS screening
  • Estrogen dominance symptoms
  • Fibrocystic breast disease
  • Osteoporosis
  • Sexual dysfunction
  • Hypogonadism

Males may consider testing for:

  • Monitor for estrogen dominance
  • Osteoporosis screening
  • Hypogonadism
  • Erectile dysfunction
  • Andropause
  • Low libido
  • Fatigue

Estradiol (E2)

Estradiol is an estrogen and the primary sex hormone for females. It is important in regulating the reproductive cycles for females. It is released from ovaries and adrenal glands and plays a major role in the growth of women's reproductive tissues, including breasts, uterus, fallopian tubes, and vagina throughout life stages. This also affects other tissues including bone, fat, skin, liver, and brain. When assessing menopausal symptoms that may include hot flashes, mood disturbances, and aging skin, it is important to compare the relationship between estradiol and progesterone. 

Males do have estradiol in their bodies but the amount compared to females is much lower. Estradiol is released from the testes and adrenal glands. Since males lack female anatomy, they must generate estrogen through a process involving aromatase, an enzyme that converts testosterone into estradiol. Estradiol has been shown in vitro to stop cell destruction, but its clinical importance in male sexual function and growth is lower than in females.

Progesterone (Pg)

Progesterone is a female hormone, released during ovulation by the ovaries. When a sperm fertilizes an egg, progesterone helps to prepare the uterus lining (endometrium) for the egg. If the egg is not fertilized, the level of progesterone decreases, and menstrual bleeding starts. During pregnancy the placenta releases high levels of progesterone, beginning at the end of the first trimester and continuing through birth. Pregnant women have progesterone levels almost 10 times higher than females who are not pregnant. Additionally, certain forms of cancer trigger elevated levels of progesterone in both men and women.


Testosterone is the primary sex hormone and anabolic steroid for males. Testosterone plays a key role in male reproductive tissue production, such as testes and prostate, as well as promoting secondary sexual characteristics, such as increased muscle and bone mass, and body hair growth. Female’s ovaries do produce testosterone in much smaller amounts compared to males. Testosterone development begins to increase dramatically during puberty and starts declining after age 30 or so.

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