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QuestAssureDâ„¢ 25-Hydroxyvitamin D (D2, D3) Blood Test, LC/MS/MS

This QuestAssureD™ 25-Hydroxyvitamin D (D2, D3) test is used to help diagnose vitamin D deficiency or toxicity and monitor response to vitamin D2 or vitamin D3 supplementation.

Sample Report

Test Code: 92888

CPT Code: 82306

Also Known As: Vitamin D2, 25-hydroxy,25-Hydroxyergocalciferol (25OHD2),Vitamin D3, 25-hydroxy,D2 Vitamin,25-Hydroxyvitamin D3,25-OH-D3,25-Hydroxycholecalciferol (25OHD3),25-OH-D2,D3 Vitamin,Vit D, LC/MS/MS,25-Hydroxyvitamin D2

Methodology: Chromatography/Mass Spectrometry

Preparation: Fasting preferred, but not required.

Test Results: 5-8 days. May take longer based on weather, holiday or lab delays.


Vitamin D3 is the main form and is produced in the skin in response to sunlight and occurs in 2 forms: vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol). Measurement of serum 25-OH vitamin D concentrations provides a good index of circulating vitamin D activity in patients not suffering from renal disease. Lower than normal 25-OH vitamin D levels can result from a dietary deficiency, poor absorption of the vitamin or impaired metabolism of the sterol in the liver. A 25-OH vitamin D deficiency can lead to bone diseases such as rickets and osteomalacia. Above normal levels can lead to hypercalcemia.

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