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Vitamin D 25-Hydroxy (D2+D3 Fractionated) Blood Test, LC/MS-MS

A Vitamin D 25-Hydroxy (D2+D3 Fractionated) Blood Test is used to measure vitamin D status, includes total 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3.

Sample Report

Test Code: 504115

Also Known As: Vitamin D, 25-Hydroxy, LC/MS-MS

Methodology: Liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Preparation: No special preparation required.

Test Results: 7-9 days. May take longer based on weather, holiday or lab delays.


25-Hydroxy vitamin D is used to measure vitamin D status; it includes results for total 25-hydroxy vitamin D2 and 25-hydroxy vitamin D3. This 25-hydroxy vitamin D assay and the alternative immunoassay test (Vitamin D, 25-Hydroxy [081950]) are both CDC-certified for accuracy.

The biological function of vitamin D is to maintain normal levels of calcium and phosphorus absorption. 25-Hydroxy vitamin D is the storage form of vitamin D. Vitamin D helps maintain bone health by facilitating calcium absorption. Deficiency of vitamin D also can cause osteomalacia, which often affects the elderly. Vitamin D from sunlight on the skin or dietary consumption is primarily converted to 25-hydroxy vitamin D by the liver, which has a long half-life and is processed in the adipose tissue. The metabolically active form of vitamin D, 1,25-di-hydroxy vitamin D, which has a short life, is then synthesized in the kidney as needed from circulating 25-hydroxy vitamin D. 

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