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Vitamin D 25-Hydroxy (D2+D3 Fractionated) Blood Test, LC/MS-MS

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The Vitamin D, 25-Hydroxy (D2+D3 Fractionated) Blood Test measures total Vitamin D and its two primary forms (D2 and D3) in the blood to screen for a deficiency or toxicity.


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Test Code:


CPT Code(s):


Also Known As:

Vitamin D, 25-Hydroxy, LC/MS-MS


Liquid Chromatography/Tandem Mass Spectrometry (LC/MS-MS)




No special preparation is required.

Test Results:

4-10 days. May take longer based on weather, holiday, or lab delays.

Walk-In Lab is prohibited from selling LabCorp tests to residents in the following states:NY, NJ, RI, MA, MD

The Vitamin D, 25-Hydroxy (D2+D3 Fractionated) Blood Test includes:

  • Total Vitamin D
  • Vitamin D2
  • Vitamin D3


What is the purpose of this test?

Order this Vitamin D, 25-Hydroxy (D2+D3 Fractionated) Blood Test to measure total Vitamin D and its two primary forms (D2 and D3) in the blood to screen for a deficiency or toxicity. 


Vitamin D is essential for supporting overall health, especially healthy bones. Vitamin D allows the body to absorb vital minerals from food and maintain adequate calcium, phosphate, magnesium, and parathyroid hormone levels in the blood. Vitamin D also has a fundamental role in muscle movement by allowing the brain and body to communicate through our nervous system. 


Additionally, research has shown that individuals with deficient vitamin D levels have increased risks of developing health problems such as:

  • Heart disease
  • Diabetes
  • Certain cancers


What does the test measure?

Vitamin D can be consumed as a nutrient in the diet and produced as a hormone in the body through direct sun exposure. Therefore, the total Vitamin D in the body is made of 2 forms:


  • Vitamin D2 (ergocalciferol) - is mainly produced in plants like yeast and mushrooms. In addition, D2 is available as a supplement and in fortified foods. 
  • Vitamin D3 (cholecalciferol) - is primarily found in animal products such as eggs, fish, and liver. D3 is also produced in the skin after exposure to direct sunlight. Like D2, D3 is offered as a dietary supplement and added to food products such as milk, juices, bread, and cereals. 


Both vitamin D2 and D3 are occasionally used to treat people with vitamin D deficiencies or related disorders, such as rickets, hypoparathyroidism, hypophosphatemia, or osteomalacia. 


Individuals should note that excessive vitamin D levels, referred to as vitamin D toxicity, can be as harmful as a deficiency. Vitamin D toxicity is caused by the overdosage of vitamin D supplements or overexposure to sunlight. Excessive amounts of vitamin D may cause damage to the kidneys and lead to elevated calcium levels in the bloodstream. This condition is known as hypercalcemia. 


This Vitamin D, 25-Hydroxy (D2+D3 Fractionated) Blood Test may assist in the screening, diagnosing, and monitoring of a vitamin D deficiency or toxicity by measuring total vitamin D and each form. A traditional Vitamin D 25-Hydroxy Blood Test that measures total Vitamin D levels is also available.


When should I get a Vitamin D, 25-Hydroxy (D2+D3 Fractionated) Blood Test?

Individuals may order this test if they have experienced signs or symptoms of a vitamin D deficiency, such as:

  • Bone or joint pain
  • Muscle weakness or aches
  • Soft, deformed, or broken bones
  • Low bone density (osteoporosis or osteopenia)


Individuals may also order this test if they have an increased risk of developing a vitamin D deficiency, such as:

  • Age over 65
  • Obese or overweight
  • History of weight loss surgery
  • Osteoporosis
  • Limited sun exposure, sunscreen use, or 
  • Dark skin pigmentation
  • A condition that causes malabsorption of nutrients from foods (celiac disease and Crohn’s disease)
  • Kidney and liver disease
  • Use certain medications

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