Quick Facts
- Sample: Blood (Morning collection /am-10am)
- Fasting: No special preparation is required.
- Turnaround: 5-7 days. May take longer based on weather, holiday, or lab delays.
What This Test Measures
This test measures two distinct testosterone biomarkers: Testosterone, Free and Testosterone, Total, MS. Free testosterone is measured using equilibrium dialysis, a method that separates and quantifies the unbound, biologically active portion of testosterone in the blood. Total testosterone is measured separately using LC/MS/MS (liquid chromatography tandem mass spectrometry), which quantifies the overall amount of testosterone present, including both bound and unbound forms.
These are two separately reported, directly measured values rather than a single combined result. The levels reported for this test are determined using different methodologies than standard free and total testosterone tests, so results from this test should not be directly compared to results from other testosterone tests using different methods.
Benefits
- Measures free testosterone using the equilibrium dialysis method
- Measures total testosterone using LC/MS/MS methodology
- Reports two testosterone values separately for clinical review
- Supports evaluation of symptoms linked to testosterone imbalance
- Provides a benchmark for monitoring testosterone levels over time
- Uses methodology suited for high-precision testosterone quantification
Who Is This Test For?
- Men experiencing decreased sex drive, erectile dysfunction, or fatigue
- Men experiencing muscle weakness potentially linked to testosterone levels
- Women with polycystic ovary syndrome (PCOS) and elevated testosterone
- Individuals undergoing dialysis with potentially altered testosterone levels
- Individuals with a pituitary or hypothalamic disorder affecting testosterone
- Athletes or bodybuilders monitoring testosterone levels
How It Works
- Order Your Test Online
- Visit a Local Lab
- Get Results Online
FAQ
1. What is the purpose of this hormone test?
This test measures two testosterone biomarkers: free testosterone (by equilibrium dialysis) and total testosterone (by LC/MS/MS). Free testosterone reflects the unbound, biologically active portion of testosterone, while total testosterone reflects the overall amount present in the blood. Together, these results may help a healthcare provider evaluate testosterone status as part of a broader clinical picture. This test does not diagnose a condition on its own.
2. Who would benefit from this test?
Men experiencing symptoms such as decreased sex drive, erectile dysfunction, fatigue, or muscle weakness may consider this test. Women with polycystic ovary syndrome (PCOS) who may have elevated testosterone may also benefit. Individuals undergoing dialysis, or those with a pituitary or hypothalamic disorder affecting testosterone production, may find this test relevant. Athletes or bodybuilders monitoring testosterone levels are also candidates for this test.
3. When should I order this test?
You may consider this test if you're experiencing symptoms like decreased sex drive, erectile dysfunction, fatigue, muscle weakness, decreased bone density, or mood changes. It may also be useful for monitoring testosterone levels if you are undergoing dialysis or have a condition affecting testosterone production. This test does not replace a full medical evaluation by a healthcare provider.
4. How does this test differ from other hormone tests?
This test directly measures free testosterone by equilibrium dialysis and total testosterone by LC/MS/MS, rather than measuring only one form. The levels reported are determined using different methodologies than standard free and total testosterone tests, so results are not directly interchangeable with those from other testosterone tests. Free testosterone reflects the biologically active, unbound fraction, while total testosterone reflects the combined bound and unbound amount.
5. Do I need to fast before this test?
No special preparation is required for this test.
6. How quickly will I get results?
Results are typically available in 5-7 days. Turnaround may take longer based on weather, holidays, or lab delays.
7. How do I interpret the results?
Interpreting free and total testosterone results depends on factors such as your symptoms, medical history, sex, age, medications, and the testing methodology used. Because this test uses equilibrium dialysis and LC/MS/MS, results may differ from those of standard testosterone tests and are often interpreted alongside other clinical findings or hormone measurements, such as luteinizing hormone (LH). One abnormal result does not automatically confirm a hormonal condition, and one normal result does not rule one out. Free and total testosterone values reflect different aspects of testosterone status and should not be treated as interchangeable.
Disclaimer: Reference ranges may vary by laboratory. Listed ranges are general guidelines and may differ from those used by the performing lab. Always consult your healthcare provider for interpretation.
8. What should I do if my results are abnormal?
If your results fall outside the expected range, review them with a qualified healthcare provider. They can help determine whether additional evaluation or testing is appropriate based on your symptoms and overall health. This test does not provide a complete clinical conclusion on its own.
9. How often should I get tested?
Testing frequency should be determined as directed by your healthcare provider, based on your individual symptoms and health status.
Pre test preparation
No special preparation is required.
