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Testosterone Total Serum Test

The Testosterone Total Serum Test measures total testosterone hormone levels in the blood to detect an abnormal level or hormone imbalance.

Sample Report

Test Code: 004226

CPT Code: 84403

Also Known As:


Electrochemiluminescence Immunoassay (ECLIA)

Specimen Type: Blood


No special preparation is required.

Test Results:

1-2 days. May take longer based on weather, holiday or lab delays.


Please note: Any result in excess of 1500 will be reported as >1500


What is the purpose of this test?

Order this Testosterone Total Serum Test to measure total testosterone hormone levels in the blood to detect an abnormal level or hormone imbalance. Testosterone are primary hormones essential to developing male physical features and reproductive functions, such as:

  • Development of body hair, muscle growth, and a deepening voice
  • Regulate sex drive, sustain muscle mass, and helps produce sperm in the testes (testicles)


It should be noted that women also make testosterone but in much smaller amounts in the ovaries and adrenal glands. There are two major forms of testosterone in the body commonly measured to help diagnose specific health problems: free and total testosterone. Free testosterone is the active or unbound form of testosterone that acts as a receptor to cells in the body. However, total testosterone includes both the unbound testosterone and bound testosterone that is attached to proteins (SHBG or albumin).


Individuals may order this Testosterone Total Serum Test to:

  • Determine the cause of early or late puberty in children and teens
  • Determine the cause of infertility 
  • Monitor infertility treatments
  • Monitor the health of transgender men taking testosterone for gender-affirming hormone therapy


What conditions cause abnormally low testosterone levels?

Several conditions may affect testosterone levels. Therefore, discussing concerns with a healthcare provider and using blood tests such as this to help monitor hormone levels is essential. For example, testosterone levels may be abnormally low due to conditions such as:

  • Thyroid problems
  • A tumor detected in the ovaries, adrenal glands, or testicles
  • Autoimmune disorders
  • Chronic or recurring illness (kidney or liver disease, lung disease)
  • Acute infections
  • Injury to the testicles


When should I order a Testosterone Total Serum Test?

Individuals may order a Testosterone Total Serum Test when if they experience symptoms associated with a testosterone deficiency or hormone imbalance, such as: 

  • Loss of sex drive
  • Erectile dysfunction or "ED"
  • Infertility
  • Enlarged breasts
  • Lack of face or body hair
  • Brittle bones or anemia without a known cause
  • Muscle weakness

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