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Testosterone, Free, Direct With Total Testosterone Blood Test, LC/MS-MS

The testosterone, free, direct with total testosterone test determines the level of free and total testosterone in a blood sample.

Sample Report

Test Code: 070195

CPT Code: 84402, 84403

Also Known As:

Methodology: Free: Direct analog enzyme immunoassay (EIA); Total: liquid chromatography/tandem mass spectrometry (LC/MS-MS)

Preparation: No special preparation required.

Test Results: 4-6 days. May take longer based on weather, holiday or lab delays.


Please note: Total testosterone levels are reported as the actual amount with this test even if the level exceeds 1500.  Free (direct) testosterone in excess of 50 will be reported as >50.


Order a testosterone, free, direct with total testosterone blood test to measure the level of free and total testosterone in the blood.  This test uses direct analog enzyme immunoassay (EIA) to report free testosterone and liquid chromatography/tandem mass spectrometry (LC/MS-MS) to report total testosterone.

Testosterone is the main male sex hormone. In both men and women, nevertheless, it is present. Testosterone is produced mainly in the testicles in men. In women, the ovaries produce small quantities of testosterone. However, it is produced by the adrenal glands in both men and women. 

Most testosterone is bound to the sex hormone-binding globulin and albumin proteins. The testosterone part that is not bound is known as free testosterone. Free testosterone is available to be used by the body. This measurement may be used to assist in the assessment of individuals when cirrhosis, thyroid disorders, or obesity may affect testosterone-binding proteins.

Some groups of individuals, such as women, children, and men whose bodies do not produce enough testosterone (hypogonadism), can find that the standard blood test for testosterone is not sufficiently responsive to give them precise results. This test may be more suitable in these situations. When testosterone concentrations are very low, this test is more sensitive and offers a more precise measurement.

This test is usually ordered to monitor testosterone levels in:

Men displaying symptoms such as:

  • Erectile dysfunction
  • Infertility
  • Loss of muscle mass
  • Osteoporosis
  • Reduced sex drive
  • Fatigue
  • Lack of body or facial hair growth
  • Delayed puberty

Women displaying symptoms such as:

  • Thinning hair
  • Acne
  • Abnormal menstrual cycles
  • Low sex drive
  • Abnormal facial or body hair growth
  • Infertility
  • Osteoporosis
  • Depression
  • Fatigue

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